Hotspot

accD-psaI

LSC · 59856-60456 · 601 bp

Back to Cathetus

Pi 0.042

MarkerSeek score 81.70

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 601
variable_sites: 24
pi: 0.042
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 81.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	AAGGGCGTTCTGAATGAGTT	TCGCAATTGCCGGAAATACT	45.0	45.0	57.4	58.3	749-791 bp	1.0	79.9
accD-psaI_p2	AAAGGGCGTTCTGAATGAGT	TCGCAATTGCCGGAAATACT	45.0	45.0	57.4	58.3	750-792 bp	1.0	79.8
accD-psaI_p3	GCTCCACGCTTTCTTTCCTT	TCGCAATTGCCGGAAATACT	50.0	45.0	58.8	58.3	723-765 bp	1.0	78.5
accD-psaI_p4	GCTCCACGCTTTCTTTCCT	TCGCAATTGCCGGAAATACT	52.6	45.0	58.1	58.3	723-765 bp	1.0	76.0
accD-psaI_p5	TCCACGCTTTCTTTCCTTTGA	TCGCAATTGCCGGAAATACT	42.9	45.0	58.1	58.3	721-763 bp	1.0	76.0