Hotspot

ndhC-trnV-UAC

LSC · 51277-52295 · 1019 bp

Back to Cathetus

Pi 0.045
MarkerSeek score 76.90
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
1019
variable_sites
42
pi
0.045
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.92
markerseek_score
76.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 TGCCCAGAAAATATCATATTCGT AGAAGGTCTACGGTTCGAGT 34.8 50.0 56.1 57.8 1088-1174 bp 1.0 47.0
ndhC-trnV-UAC_p2 TGCCCAGAAAATATCATATTCGT GAAGGTCTACGGTTCGAGTC 34.8 55.0 56.1 57.5 1087-1173 bp 1.0 47.0
ndhC-trnV-UAC_p3 TGCCCAGAAAATATCATATTCGT GTTCGAGTCCGTATAGCCCT 34.8 55.0 56.1 58.7 1076-1162 bp 1.0 47.0
ndhC-trnV-UAC_p4 ATGCCCAGAAAATATCATATTCGT AGAAGGTCTACGGTTCGAGT 33.3 50.0 56.6 57.8 1089-1175 bp 1.0 47.0
ndhC-trnV-UAC_p5 TGCCCAGAAAATATCATATTCGT AAGGTCTACGGTTCGAGTCC 34.8 55.0 56.1 58.8 1086-1172 bp 1.0 47.0