Hotspot

psbZ-trnG-GCC

LSC · 37255-37863 · 609 bp

Back to Cathetus

Pi 0.0874

MarkerSeek score 88.00

Primer available yes

Label psbZ-trnG-GCC

Feature Metadata

label_name: psbZ-trnG-GCC
feature_type: igs
region: LSC
length_bp: 609
variable_sites: 50
pi: 0.0874
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 88.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbZ-trnG-GCC_p1	TCCTGATGGTTGGTCAAGTAAC	CGTCTTCTCCTTGGCAAAGA	45.5	50.0	58.0	57.8	759-874 bp	1.0	71.0
psbZ-trnG-GCC_p2	TCCTGATGGTTGGTCAAGTA	CGTCTTCTCCTTGGCAAAGA	45.0	50.0	55.8	57.8	759-874 bp	1.0	69.3
psbZ-trnG-GCC_p3	CCTGATGGTTGGTCAAGTAACA	CGTCTTCTCCTTGGCAAAGA	45.5	50.0	58.2	57.8	758-873 bp	1.0	69.2
psbZ-trnG-GCC_p4	TCCTGATGGTTGGTCAAGTAA	CGTCTTCTCCTTGGCAAAGA	42.9	50.0	56.6	57.8	759-874 bp	1.0	67.7
psbZ-trnG-GCC_p5	CCTGATGGTTGGTCAAGTAAC	CGTCTTCTCCTTGGCAAAGA	47.6	50.0	56.5	57.8	758-873 bp	1.0	67.4