Hotspot

trnD-GUC-trnY-GUA

LSC · 30681-31097 · 417 bp

Back to Cathetus

Pi 0.0539
MarkerSeek score 77.60
Primer available yes
Label trnD-GUC-trnY-GUA

Feature Metadata

label_name
trnD-GUC-trnY-GUA
feature_type
igs
region
LSC
length_bp
417
variable_sites
22
pi
0.0539
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.98
markerseek_score
77.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnD-GUC-trnY-GUA_p1 TTTGGATCTGTCGGGACTGA GGGGACGGACTGTAAATTCG 50.0 55.0 58.4 58.4 563-579 bp 1.0 78.6
trnD-GUC-trnY-GUA_p2 TTTGGATCTGTCGGGACTGA GGGACGGACTGTAAATTCGT 50.0 50.0 58.4 57.6 562-578 bp 1.0 78.5
trnD-GUC-trnY-GUA_p3 CGGTGCTCTGACCAATTGAA GGGGACGGACTGTAAATTCG 50.0 55.0 58.5 58.4 508-524 bp 1.0 78.2
trnD-GUC-trnY-GUA_p4 CGGTGCTCTGACCAATTGAA GGGACGGACTGTAAATTCGT 50.0 50.0 58.5 57.6 507-523 bp 1.0 78.0
trnD-GUC-trnY-GUA_p5 TTTGGATCTGTCGGGACTGA GATTCTTCCTGGGTCGATGC 50.0 55.0 58.4 58.4 596-612 bp 1.0 77.9