Hotspot

trnS-GCU-trnG-UCC

LSC · 9242-10013 · 772 bp

Back to Cathetus

Pi 0.0517
MarkerSeek score 82.50
Primer available yes
Label trnS-GCU-trnG-UCC

Feature Metadata

label_name
trnS-GCU-trnG-UCC
feature_type
igs
region
LSC
length_bp
772
variable_sites
39
pi
0.0517
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.98
markerseek_score
82.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-GCU-trnG-UCC_p1 CAATCCGACGCTTTAGTCCA TGAACCGAGAGATCCCTTAAC 50.0 47.6 58.0 56.8 878-907 bp 1.0 67.7
trnS-GCU-trnG-UCC_p2 AATCCGACGCTTTAGTCCAC TGAACCGAGAGATCCCTTAAC 50.0 47.6 58.0 56.8 877-906 bp 1.0 67.7
trnS-GCU-trnG-UCC_p3 CAATCCGACGCTTTAGTCCA TGAACCGAGAGATCCCTTAACT 50.0 45.5 58.0 58.3 878-907 bp 1.0 66.7
trnS-GCU-trnG-UCC_p4 AATCCGACGCTTTAGTCCAC TGAACCGAGAGATCCCTTAACT 50.0 45.5 58.0 58.3 877-906 bp 1.0 66.7
trnS-GCU-trnG-UCC_p5 AGAACCGGAAAGAGAGGGAT TGAACCGAGAGATCCCTTAAC 50.0 47.6 57.7 56.8 936-965 bp 1.0 65.4