Hotspot

rpoA

LSC · 72479-73504 · 1026 bp

Back to Catapodium

Pi 0.0013

MarkerSeek score 47.20

Primer available yes

Label rpoA

Feature Metadata

label_name: rpoA
feature_type: gene
region: LSC
length_bp: 1026
variable_sites: 2
pi: 0.0013
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 47.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoA_p1	GTTCCTTTGGGGGATGGATC	CCATTGCTAAAAGCGGTGTG	55.0	50.0	57.9	58.0	1912 bp	1.0	79.4
rpoA_p2	GTTCCTTTGGGGGATGGATC	ATGGTAAAAGGCGCTGGTAG	55.0	50.0	57.9	58.0	1955 bp	1.0	79.2
rpoA_p3	GTTCCTTTGGGGGATGGATC	GTGGAAGAGATGCCGCATTA	55.0	50.0	57.9	58.0	1936 bp	1.0	79.2
rpoA_p4	GTTCCTTTGGGGGATGGATC	TGGAAGAGATGCCGCATTAC	55.0	50.0	57.9	58.0	1935 bp	1.0	79.2
rpoA_p5	GTTCCTTTGGGGGATGGATC	GATGTAACACCTATGCCGCA	55.0	50.0	57.9	58.1	1874 bp	1.0	79.1