Hotspot

ndhA

SSC · 109711-111825 · 2115 bp

Back to Catapodium

Pi 0.00063

MarkerSeek score 57.90

Primer available yes

Label ndhA

Feature Metadata

label_name: ndhA
feature_type: gene
region: SSC
length_bp: 2115
variable_sites: 2
pi: 0.00063
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhA_p1	ACCCAGTTACCATAGGGAACA	TATGGGGGAAGTTGACCGTT	47.6	50.0	58.1	58.6	2257 bp	1.0	66.4
ndhA_p2	ACCCAGTTACCATAGGGAACA	TTATGGGGGAAGTTGACCGT	47.6	50.0	58.1	58.6	2258 bp	1.0	66.4
ndhA_p3	ACCCAGTTACCATAGGGAACA	ATTATGGGGGAAGTTGACCG	47.6	50.0	58.1	57.3	2259 bp	1.0	65.6
ndhA_p4	ACCCAGTTACCATAGGGAACA	TATGGGGGAAGTTGACCGT	47.6	52.6	58.1	57.9	2257 bp	1.0	62.7
ndhA_p5	CCCAGTTACCATAGGGAACA	TATGGGGGAAGTTGACCGTT	50.0	50.0	56.2	58.6	2256 bp	1.0	60.7