Hotspot

ndhF-rpl32

SSC · 102442-103304 · 863 bp

Back to Catapodium

Pi 0.00232

MarkerSeek score 60.20

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 863
variable_sites: 3
pi: 0.00232
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	GGAAGTGGGAGAAGAGGGAT	TGCTAAAGAATAAGCGTGTACT	55.0	36.4	58.1	55.6	1018 bp	1.0	44.0
ndhF-rpl32_p2	GGCCAAACCCCATTGACATA	TGCTAAAGAATAAGCGTGTACT	50.0	36.4	58.1	55.6	1044 bp	1.0	43.8
ndhF-rpl32_p3	AAAAGGCCAAACCCCATTGA	TGCTAAAGAATAAGCGTGTACT	45.0	36.4	58.2	55.6	1048 bp	1.0	43.4
ndhF-rpl32_p4	GGAAGTGGGAGAAGAGGGAT	TTGCTAAAGAATAAGCGTGTACT	55.0	34.8	58.1	56.3	1019 bp	1.0	41.7
ndhF-rpl32_p5	GGCCAAACCCCATTGACATA	TTGCTAAAGAATAAGCGTGTACT	50.0	34.8	58.1	56.3	1045 bp	1.0	41.5