Hotspot

petA-psbJ

LSC · 59096-59943 · 848 bp

Back to Catapodium

Pi 0.00472

MarkerSeek score 61.80

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 848
variable_sites: 6
pi: 0.00472
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	ATCCATTACGTGTCCAAGGC	ACGGTTCATATTCTGGATTGGG	50.0	45.5	58.0	58.2	1002 bp	1.0	62.7
petA-psbJ_p2	ATCCATTACGTGTCCAAGGC	CGGTTCATATTCTGGATTGGGT	50.0	45.5	58.0	58.2	1001 bp	1.0	62.7
petA-psbJ_p3	TTTGTTTTTCTTCGCGTCCG	ACGGTTCATATTCTGGATTGGG	45.0	45.5	57.9	58.2	980 bp	1.0	62.3
petA-psbJ_p4	TTTGTTTTTCTTCGCGTCCG	CGGTTCATATTCTGGATTGGGT	45.0	45.5	57.9	58.2	979 bp	1.0	62.3
petA-psbJ_p5	CTTCGCGTCCGTTATTTTGG	CGGTTCATATTCTGGATTGGGT	50.0	45.5	57.8	58.2	970 bp	1.0	61.8