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trnC-GCA-rpoB

LSC · 16846-18083 · 1238 bp

Back to Catapodium

Pi 0.00108

MarkerSeek score 63.20

Primer available yes

Label trnC-GCA-rpoB

Feature Metadata

label_name: trnC-GCA-rpoB
feature_type: igs
region: LSC
length_bp: 1238
variable_sites: 2
pi: 0.00108
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-rpoB_p1	AAAGGATTTGCAGTCCCCTG	ATTCCCTCGTTTCCATTCCG	50.0	50.0	58.1	58.0	1266-1307 bp	1.0	81.8
trnC-GCA-rpoB_p2	AAAGGATTTGCAGTCCCCTG	TGTGGACATTCCCTCGTTTC	50.0	50.0	58.1	57.8	1273-1314 bp	1.0	80.6
trnC-GCA-rpoB_p3	AAAGGATTTGCAGTCCCCTG	GTTCTTCTGCCAGGGCTTTA	50.0	50.0	58.1	57.8	1343-1384 bp	1.0	80.3
trnC-GCA-rpoB_p4	AAAGGATTTGCAGTCCCCTG	TGTCAAGTTCTTCTGCCAGG	50.0	50.0	58.1	57.7	1349-1390 bp	1.0	79.8
trnC-GCA-rpoB_p5	AAAGGATTTGCAGTCCCCTG	CGGGTATTGTGGACATTCCC	50.0	55.0	58.1	58.3	1280-1321 bp	1.0	79.5