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trnK-UUU-rps16

LSC · 3956-4512 · 557 bp

Back to Catapodium

Pi 0.00239

MarkerSeek score 59.40

Primer available yes

Label trnK-UUU-rps16

Feature Metadata

label_name: trnK-UUU-rps16
feature_type: igs
region: LSC
length_bp: 557
variable_sites: 2
pi: 0.00239
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rps16_p1	TGGACGAATTTGTTGCTCCA	TCAACCGACAAGAACCGTTT	45.0	45.0	58.0	58.0	719 bp	1.0	79.8
trnK-UUU-rps16_p2	TGGACGAATTTGTTGCTCCA	CTCAACCGACAAGAACCGTT	45.0	50.0	58.0	58.4	720 bp	1.0	76.6
trnK-UUU-rps16_p3	AGTCGCACTTAAAAGCCGAG	TCAACCGACAAGAACCGTTT	50.0	45.0	58.6	58.0	678 bp	1.0	75.5
trnK-UUU-rps16_p4	CTGGACGAATTTGTTGCTCC	TCAACCGACAAGAACCGTTT	50.0	45.0	57.4	58.0	720 bp	1.0	75.1
trnK-UUU-rps16_p5	TCGCACTTAAAAGCCGAGTA	TCAACCGACAAGAACCGTTT	45.0	45.0	57.3	58.0	676 bp	1.0	74.2