Hotspot

psbC

LSC · 34130-35551 · 1422 bp

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Pi 0.00169

MarkerSeek score 52.40

Primer available yes

Label psbC

Feature Metadata

label_name: psbC
feature_type: gene
region: LSC
length_bp: 1422
variable_sites: 5
pi: 0.00169
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 52.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbC_p1	GTTTCTCAGGAAATTCGCGC	ACCTGATCCCAATATGTCTGA	50.0	42.9	58.1	55.8	1608 bp	0.8	45.8
psbC_p2	CGTTTCTCAGGAAATTCGCG	ACCTGATCCCAATATGTCTGA	50.0	42.9	57.9	55.8	1609 bp	0.8	45.6
psbC_p3	GTTTCTCAGGAAATTCGCGC	ACCTGATCCCAATATGTCTGATG	50.0	43.5	58.1	57.8	1608 bp	0.8	45.3
psbC_p4	CGTTTCTCAGGAAATTCGCG	ACCTGATCCCAATATGTCTGATG	50.0	43.5	57.9	57.8	1609 bp	0.8	45.1
psbC_p5	AGGTATTCGTGCTTGGATGG	ACCTGATCCCAATATGTCTGA	50.0	42.9	57.7	55.8	1534 bp	0.8	44.1