Hotspot

ndhF

SSC · 115356-117584 · 2229 bp

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Pi 0.00817

MarkerSeek score 41.00

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2229
variable_sites: 39
pi: 0.00817
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 41.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	GAAAACGTCAACGACTGCTG	TTAACGCTGTCCAATACCCC	50.0	50.0	58.0	57.6	2866-2873 bp	0.8	69.8
ndhF_p2	GAAAACGTCAACGACTGCTG	TAACGCTGTCCAATACCCCT	50.0	50.0	58.0	58.4	2865-2872 bp	0.8	69.6
ndhF_p3	TTGGGAAAACGTCAACGACT	TTAACGCTGTCCAATACCCC	45.0	50.0	58.0	57.6	2870-2877 bp	0.8	69.6
ndhF_p4	TTGGGAAAACGTCAACGACT	TAACGCTGTCCAATACCCCT	45.0	50.0	58.0	58.4	2869-2876 bp	0.8	69.4
ndhF_p5	CATGTCAGAGAGGTTTGCGA	TTAACGCTGTCCAATACCCC	50.0	50.0	57.9	57.6	2903 bp	0.4	52.1