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clpP-psbB

LSC · 73000-73448 · 449 bp

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Pi 0.000891

MarkerSeek score 47.20

Primer available yes

Label clpP-psbB

Feature Metadata

label_name: clpP-psbB
feature_type: igs
region: LSC
length_bp: 449
variable_sites: 1
pi: 0.000891
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 47.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP-psbB_p1	TATACGTCAGCCCAAGTTGC	CGATACCAAGGCAAACCCAT	50.0	50.0	58.0	58.2	517-540 bp	1.0	81.5
clpP-psbB_p2	TTTGGAACACCAACAGGCAT	CGATACCAAGGCAAACCCAT	45.0	50.0	58.2	58.2	466-489 bp	1.0	80.4
clpP-psbB_p3	CTTTTGGAACACCAACAGGC	CGATACCAAGGCAAACCCAT	50.0	50.0	57.8	58.2	468-491 bp	1.0	80.3
clpP-psbB_p4	AAGTCGCACTATACGTCAGC	CGATACCAAGGCAAACCCAT	50.0	50.0	57.8	58.2	526-549 bp	1.0	80.1
clpP-psbB_p5	TATACGTCAGCCCAAGTTGC	ACACGATACCAAGGCAAACC	50.0	50.0	58.0	58.5	520-543 bp	1.0	79.7