Hotspot

psaA-ycf3

LSC · 42064-42817 · 754 bp

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Pi 0.00292

MarkerSeek score 47.20

Primer available yes

Label psaA-ycf3

Feature Metadata

label_name: psaA-ycf3
feature_type: igs
region: LSC
length_bp: 754
variable_sites: 5
pi: 0.00292
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 47.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-ycf3_p1	TGAGAAATGACCCGGTTTGG	GGAAACAGGCTATAGCGCTT	50.0	50.0	58.1	58.0	924-933 bp	1.0	80.1
psaA-ycf3_p2	CCCATGCCTCGAAAGAAGTT	GGAAACAGGCTATAGCGCTT	50.0	50.0	58.2	58.0	904-913 bp	1.0	79.5
psaA-ycf3_p3	TGAGAAATGACCCGGTTTGG	TTGAAGATCACGAGGCGTTT	50.0	45.0	58.1	57.8	871-880 bp	1.0	79.2
psaA-ycf3_p4	TGAGAAATGACCCGGTTTGG	TTGGTTGAAGATCACGAGGC	50.0	50.0	58.1	58.2	875-884 bp	1.0	79.0
psaA-ycf3_p5	CCCATGCCTCGAAAGAAGTT	TTGAAGATCACGAGGCGTTT	50.0	45.0	58.2	57.8	851-860 bp	1.0	78.6