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petN-psbM

LSC · 29444-30435 · 992 bp

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Pi 0.00444

MarkerSeek score 47.30

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 992
variable_sites: 9
pi: 0.00444
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 47.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	ACTCGTAGTGTGGGGAAGAA	AGTTCCTACCGCTTTTCTACT	50.0	42.9	58.0	56.4	1081-1087 bp	1.0	60.1
petN-psbM_p2	TCGTAGTGTGGGGAAGAAGT	AGTTCCTACCGCTTTTCTACT	50.0	42.9	58.0	56.4	1079-1085 bp	1.0	60.1
petN-psbM_p3	ACTCGTAGTGTGGGGAAGAA	TCATTCTAGTTCCTACCGCT	50.0	45.0	58.0	55.4	1088-1094 bp	1.0	59.9
petN-psbM_p4	TCGTAGTGTGGGGAAGAAGT	TCATTCTAGTTCCTACCGCT	50.0	45.0	58.0	55.4	1086-1092 bp	1.0	59.9
petN-psbM_p5	CGTAGTGTGGGGAAGAAGTG	AGTTCCTACCGCTTTTCTACT	55.0	42.9	57.9	56.4	1078-1084 bp	1.0	59.5