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trnH-GUG-psbA

LSC · 98-385 · 288 bp

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Pi 0.00833

MarkerSeek score 29.80

Primer available yes

Label trnH-GUG-psbA

Feature Metadata

label_name: trnH-GUG-psbA
feature_type: igs
region: LSC
length_bp: 288
variable_sites: 5
pi: 0.00833
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 29.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	GATCCACTTGGCTACATCCG	GAGCTTAGTTTCCGTCTGGG	55.0	55.0	58.1	58.0	976-991 bp	1.0	79.8
trnH-GUG-psbA_p2	GATCCACTTGGCTACATCCG	GAGTGGGAGCTTAGTTTCCG	55.0	55.0	58.1	58.0	982-997 bp	1.0	79.8
trnH-GUG-psbA_p3	GATCCACTTGGCTACATCCG	GGAGCTTAGTTTCCGTCTGG	55.0	55.0	58.1	58.0	977-992 bp	1.0	79.8
trnH-GUG-psbA_p4	GATCCACTTGGCTACATCCG	GGGAGCTTAGTTTCCGTCTG	55.0	55.0	58.1	58.0	978-993 bp	1.0	79.8
trnH-GUG-psbA_p5	CAATCCACTGCCTTGATCCA	GAGCTTAGTTTCCGTCTGGG	50.0	55.0	57.9	58.0	990-1005 bp	1.0	79.7