Hotspot

trnV-UAC

LSC · 53579-53613 · 35 bp

Back to Casuarina

Pi 0.019

MarkerSeek score 52.10

Primer available yes

Label trnV-UAC

Feature Metadata

label_name: trnV-UAC
feature_type: tRNA
region: LSC
length_bp: 35
variable_sites: 1
pi: 0.019
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 52.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnV-UAC_p1	GGCACATGTCACGTTTCAAG	CGGGCCAAAAGCATACCTAT	50.0	50.0	58.0	58.0	528 bp	1.0	79.7
trnV-UAC_p2	GGCACATGTCACGTTTCAAG	ACAAGCACAAGGGCTATAGC	50.0	50.0	58.0	58.0	955 bp	1.0	79.4
trnV-UAC_p3	ACAAATAAAGCTCTCGCGGT	CGGGCCAAAAGCATACCTAT	45.0	50.0	57.9	58.0	462 bp	1.0	79.3
trnV-UAC_p4	ACAAATAAAGCTCTCGCGGT	ACAAGCACAAGGGCTATAGC	45.0	50.0	57.9	58.0	889 bp	1.0	79.0
trnV-UAC_p5	GGGGCTAAAACTCCCGAAAT	CGGGCCAAAAGCATACCTAT	50.0	50.0	57.9	58.0	763 bp	1.0	78.9