Hotspot

ndhF

SSC · 111947-114205 · 2259 bp

Back to Casuarina

Pi 0.00207

MarkerSeek score 43.60

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2259
variable_sites: 7
pi: 0.00207
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 43.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	AAAAGAACAACATAAGCAACA	AGCTGTTGGATCGGAAATAGT	28.6	42.9	52.4	57.1	2404-2417 bp	1.0	40.2
ndhF_p2	AAAGAACAACATAAGCAACAA	AGCTGTTGGATCGGAAATAGT	28.6	42.9	52.4	57.1	2403-2416 bp	1.0	40.2
ndhF_p3	AAGAACAACATAAGCAACAAA	AGCTGTTGGATCGGAAATAGT	28.6	42.9	52.4	57.1	2402-2415 bp	1.0	40.2
ndhF_p4	AAAAGAACAACATAAGCAACAA	AGCTGTTGGATCGGAAATAGT	27.3	42.9	53.3	57.1	2404-2417 bp	1.0	40.2
ndhF_p5	AAAGAACAACATAAGCAACAAA	AGCTGTTGGATCGGAAATAGT	27.3	42.9	53.3	57.1	2403-2416 bp	1.0	40.2