Hotspot

accD

LSC · 59610-61130 · 1521 bp

Back to Casuarina

Pi 0.000438

MarkerSeek score 34.60

Primer available yes

Label accD

Feature Metadata

label_name: accD
feature_type: gene
region: LSC
length_bp: 1521
variable_sites: 1
pi: 0.000438
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 34.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD_p1	TGGATCCTTAGGGTTGGTGT	CTCGTTTTATTCGCCTTGCC	50.0	50.0	57.9	57.8	2313-2321 bp	1.0	79.0
accD_p2	TGGATCCTTAGGGTTGGTGT	TCGTTTTATTCGCCTTGCCT	50.0	45.0	57.9	58.2	2312-2320 bp	1.0	78.9
accD_p3	ATTGTTGTGTTGGGTCCACA	CTCGTTTTATTCGCCTTGCC	45.0	50.0	57.8	57.8	2343-2351 bp	1.0	78.2
accD_p4	ATTGTTGTGTTGGGTCCACA	TCGTTTTATTCGCCTTGCCT	45.0	45.0	57.8	58.2	2342-2350 bp	1.0	78.1
accD_p5	ACTTCTTCTACCCATCCCGT	CTCGTTTTATTCGCCTTGCC	50.0	50.0	57.8	57.8	2243-2251 bp	1.0	77.4