Hotspot

trnN-GUU-trnR-ACG

IRa · 132224-132842 · 619 bp

Back to Casuarina

Pi 0.00325

MarkerSeek score 61.30

Primer available yes

Label trnN-GUU-trnR-ACG

Feature Metadata

label_name: trnN-GUU-trnR-ACG
feature_type: igs
region: IRa
length_bp: 619
variable_sites: 3
pi: 0.00325
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnN-GUU-trnR-ACG_p1	CCGTTGTTCCTCAGTAGCTC	CAGAGGATTAGAGCACGTGG	55.0	55.0	58.0	57.8	757-760 bp	1.0	78.9
trnN-GUU-trnR-ACG_p2	ACTGACTGGTCGTAGGTTCA	CAGAGGATTAGAGCACGTGG	50.0	55.0	58.0	57.8	714-717 bp	1.0	78.9
trnN-GUU-trnR-ACG_p3	CCGTTGTTCCTCAGTAGCTC	GGATACTCCCGGGAACAGAT	55.0	55.0	58.0	58.3	806-809 bp	1.0	78.3
trnN-GUU-trnR-ACG_p4	ACTGACTGGTCGTAGGTTCA	GGATACTCCCGGGAACAGAT	50.0	55.0	58.0	58.3	763-766 bp	1.0	78.3
trnN-GUU-trnR-ACG_p5	TGGGTTGAGAACGGGAATTG	CAGAGGATTAGAGCACGTGG	50.0	55.0	58.1	57.8	798-801 bp	1.0	78.1