Hotspot

rpl32-trnL-UAG

SSC · 115251-116103 · 853 bp

Back to Casuarina

Pi 0.00315
MarkerSeek score 47.20
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
853
variable_sites
4
pi
0.00315
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
47.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 ACTAGAGGGTTATTGGGTAGCA AGCGTGTCTACCGATTTCAC 45.5 50.0 58.0 58.0 973-979 bp 1.0 64.8
rpl32-trnL-UAG_p2 ACTAGAGGGTTATTGGGTAGCA CAGCGTGTCTACCGATTTCA 45.5 50.0 58.0 58.0 974-980 bp 1.0 64.8
rpl32-trnL-UAG_p3 ACTAGAGGGTTATTGGGTAGCA TCCTAAGAGCAGCGTGTCTA 45.5 50.0 58.0 57.9 983-989 bp 1.0 63.9
rpl32-trnL-UAG_p4 GCGAAATCTCTTTCTACAGGGA AGCGTGTCTACCGATTTCAC 45.5 50.0 57.9 58.0 933-939 bp 1.0 63.9
rpl32-trnL-UAG_p5 GCGAAATCTCTTTCTACAGGGA CAGCGTGTCTACCGATTTCA 45.5 50.0 57.9 58.0 934-940 bp 1.0 63.9