Hotspot

ndhF-rpl32

SSC · 114206-115091 · 886 bp

Back to Casuarina

Pi 0.00903

MarkerSeek score 58.50

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 886
variable_sites: 12
pi: 0.00903
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	TGTTGCCTTGGGGAAAAGAA	TGCTACCCAATAACCCTCTAGT	45.0	45.5	57.8	58.0	1069-1070 bp	1.0	63.5
ndhF-rpl32_p2	TTGTTGCCTTGGGGAAAAGA	TGCTACCCAATAACCCTCTAGT	45.0	45.5	57.8	58.0	1070-1071 bp	1.0	63.5
ndhF-rpl32_p3	TGTTGCCTTGGGGAAAAGAA	TCAATGCTACCCAATAACCCTC	45.0	45.5	57.8	57.8	1073-1074 bp	1.0	62.2
ndhF-rpl32_p4	TTGTTGCCTTGGGGAAAAGA	TCAATGCTACCCAATAACCCTC	45.0	45.5	57.8	57.8	1074-1075 bp	1.0	62.2
ndhF-rpl32_p5	TGTTGCCTTGGGGAAAAGAA	CAATGCTACCCAATAACCCTCT	45.0	45.5	57.8	57.8	1072-1073 bp	1.0	62.2