Hotspot

rps3-rps19

LSC · 85359-85961 · 603 bp

Back to Casuarina

Pi 0.00553

MarkerSeek score 40.50

Primer available yes

Label rps3-rps19

Feature Metadata

label_name: rps3-rps19
feature_type: igs
region: LSC
length_bp: 603
variable_sites: 5
pi: 0.00553
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 40.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps3-rps19_p1	TGCACCAAGTCTGAAACCAA	CACCTACGCTGAATTTCCGA	45.0	50.0	57.9	58.0	701 bp	1.0	79.4
rps3-rps19_p2	TTGCACCAAGTCTGAAACCA	CACCTACGCTGAATTTCCGA	45.0	50.0	57.9	58.0	702 bp	1.0	79.4
rps3-rps19_p3	TGCACCAAGTCTGAAACCAA	AATTCGCACCTACGCTGAAT	45.0	45.0	57.9	58.0	707 bp	1.0	79.3
rps3-rps19_p4	TGCACCAAGTCTGAAACCAA	ATTCGCACCTACGCTGAATT	45.0	45.0	57.9	58.0	706 bp	1.0	79.3
rps3-rps19_p5	TTGCACCAAGTCTGAAACCA	ATTCGCACCTACGCTGAATT	45.0	45.0	57.9	58.0	707 bp	1.0	79.3