Hotspot

trnP-UGG-psaJ

LSC · 68495-68874 · 380 bp

Back to Casuarina

Pi 0.00175
MarkerSeek score 55.60
Primer available yes
Label trnP-UGG-psaJ

Feature Metadata

label_name
trnP-UGG-psaJ
feature_type
igs
region
LSC
length_bp
380
variable_sites
1
pi
0.00175
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
55.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnP-UGG-psaJ_p1 ACCCGTGACATTTTGTACCC AACAACGCATCCGGGAATAA 50.0 45.0 58.1 57.9 547-548 bp 1.0 78.6
trnP-UGG-psaJ_p2 ACCCGTGACATTTTGTACCC AATAACAACGCATCCGGGAA 50.0 45.0 58.1 57.9 550-551 bp 1.0 78.6
trnP-UGG-psaJ_p3 ACCCGTGACATTTTGTACCC ACAACGCATCCGGGAATAAA 50.0 45.0 58.1 57.9 546-547 bp 1.0 78.6
trnP-UGG-psaJ_p4 CCCGTGACATTTTGTACCCA AACAACGCATCCGGGAATAA 50.0 45.0 58.1 57.9 546-547 bp 1.0 78.5
trnP-UGG-psaJ_p5 CCCGTGACATTTTGTACCCA ACAACGCATCCGGGAATAAA 50.0 45.0 58.1 57.9 545-546 bp 1.0 78.5