Hotspot

rps2-rpoC2

LSC · 17105-17325 · 221 bp

Back to Casuarina

Pi 0.00603

MarkerSeek score 49.60

Primer available yes

Label rps2-rpoC2

Feature Metadata

label_name: rps2-rpoC2
feature_type: igs
region: LSC
length_bp: 221
variable_sites: 2
pi: 0.00603
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 49.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps2-rpoC2_p1	GGTGCCATTCTAGGATTCCA	CCCAAACAATCATTGCGAGG	50.0	50.0	57.0	57.7	355-356 bp	1.0	70.0
rps2-rpoC2_p2	CCTGCTTCCATCATCTCTTCC	CCCAAACAATCATTGCGAGG	52.4	50.0	58.2	57.7	307-308 bp	1.0	69.0
rps2-rpoC2_p3	GGTGCCATTCTAGGATTCCA	ACACCCAAACAATCATTGCG	50.0	45.0	57.0	57.6	358-359 bp	1.0	68.7
rps2-rpoC2_p4	TGACCAAAATGAACTCCTGCT	CCCAAACAATCATTGCGAGG	42.9	50.0	57.8	57.7	322-323 bp	1.0	68.4
rps2-rpoC2_p5	ACTCCTGCTTCCATCATCTCT	CCCAAACAATCATTGCGAGG	47.6	50.0	58.2	57.7	310-311 bp	1.0	68.3