Hotspot

rps16-trnQ-UUG

LSC · 6292-6887 · 596 bp

Back to Casuarina

Pi 0.00503

MarkerSeek score 54.00

Primer available yes

Label rps16-trnQ-UUG

Feature Metadata

label_name: rps16-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 596
variable_sites: 3
pi: 0.00503
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	CACAGCCGACCATATCCTTC	GAGGTTCGAATCCTTCCGTC	55.0	55.0	58.1	58.1	266-689 bp	1.0	82.8
rps16-trnQ-UUG_p2	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	45.0	55.0	58.1	58.1	246-669 bp	1.0	82.7
rps16-trnQ-UUG_p3	GTAAGAATCCACAGCCGACC	GAGGTTCGAATCCTTCCGTC	55.0	55.0	58.3	58.1	275-698 bp	1.0	81.0
rps16-trnQ-UUG_p4	TGTAAGAATCCACAGCCGAC	GAGGTTCGAATCCTTCCGTC	50.0	55.0	57.6	58.1	276-699 bp	1.0	80.8
rps16-trnQ-UUG_p5	ACAGCCGACCATATCCTTCA	GAGGTTCGAATCCTTCCGTC	50.0	55.0	58.5	58.1	265-688 bp	1.0	79.8