Hotspot

trnH-GUG-psbA

LSC · 83-381 · 299 bp

Back to Casuarina

Pi 0.00892

MarkerSeek score 36.40

Primer available yes

Label trnH-GUG-psbA

Feature Metadata

label_name: trnH-GUG-psbA
feature_type: igs
region: LSC
length_bp: 299
variable_sites: 4
pi: 0.00892
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 36.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	GATCCACTTGGCTACATCCG	GCGCTAACCTTGGTATGGAA	55.0	50.0	58.1	58.0	416-418 bp	1.0	79.5
trnH-GUG-psbA_p2	CAATCCACTGCCTTGATCCA	GCGCTAACCTTGGTATGGAA	50.0	50.0	57.9	58.0	430-432 bp	1.0	79.4
trnH-GUG-psbA_p3	AATCCACTGCCTTGATCCAC	GCGCTAACCTTGGTATGGAA	50.0	50.0	57.9	58.0	429-431 bp	1.0	79.4
trnH-GUG-psbA_p4	ACAATCCACTGCCTTGATCC	GCGCTAACCTTGGTATGGAA	50.0	50.0	57.9	58.0	431-433 bp	1.0	79.4
trnH-GUG-psbA_p5	CGCATGGTGGATTCACAATC	GCGCTAACCTTGGTATGGAA	50.0	50.0	57.5	58.0	445-447 bp	1.0	76.6