Hotspot

ycf3

LSC · 47231-49225 · 1995 bp

Back to Castanopsis

Pi 0.00217

MarkerSeek score 45.60

Primer available yes

Label ycf3

Feature Metadata

label_name: ycf3
feature_type: gene
region: LSC
length_bp: 1995
variable_sites: 11
pi: 0.00217
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 45.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf3_p1	TGGGGACAACAAACAAAACA	TGTGGGATCATTTTCTCGCA	40.0	45.0	56.2	57.5	2158-2167 bp	1.0	62.4
ycf3_p2	TGGGGACAACAAACAAAACAA	TGTGGGATCATTTTCTCGCA	38.1	45.0	57.0	57.5	2158-2167 bp	1.0	60.5
ycf3_p3	TGGGGACAACAAACAAAACA	TGTGGGATCATTTTCTCGCAA	40.0	42.9	56.2	58.2	2158-2167 bp	1.0	56.6
ycf3_p4	TGGGGACAACAAACAAAACA	TTGTGGGATCATTTTCTCGCA	40.0	42.9	56.2	58.2	2159-2168 bp	1.0	56.6
ycf3_p5	TGGGGACAACAAACAAAACAAT	TGTGGGATCATTTTCTCGCA	36.4	45.0	57.4	57.5	2158-2167 bp	1.0	56.4