Hotspot

ndhE-ndhG

SSC · 124397-124653 · 257 bp

Back to Castanopsis

Pi 0.0126

MarkerSeek score 57.70

Primer available yes

Label ndhE-ndhG

Feature Metadata

label_name: ndhE-ndhG
feature_type: igs
region: SSC
length_bp: 257
variable_sites: 8
pi: 0.0126
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 57.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhE-ndhG_p1	AGGCACTCACAACAAGTACA	TTGCTGTGGCTCGTCAATAA	45.0	45.0	57.0	57.8	306-332 bp	1.0	76.6
ndhE-ndhG_p2	AGGCACTCACAACAAGTACA	AGCTGCTTTAATAGGCGCAA	45.0	45.0	57.0	58.2	326-352 bp	1.0	75.9
ndhE-ndhG_p3	AGGCACTCACAACAAGTACA	ATTGCTGTGGCTCGTCAATA	45.0	45.0	57.0	57.6	307-333 bp	1.0	74.8
ndhE-ndhG_p4	AGGCACTCACAACAAGTACA	AATTGCTGTGGCTCGTCAAT	45.0	45.0	57.0	58.5	308-334 bp	1.0	74.3
ndhE-ndhG_p5	CGAGCATCATTGAACAACTCC	TTGCTGTGGCTCGTCAATAA	47.6	45.0	57.6	57.8	282-308 bp	1.0	73.6