Hotspot

rpl16-rps3

LSC · 87474-88725 · 1252 bp

Back to Castanopsis

Pi 0.00317
MarkerSeek score 58.00
Primer available yes
Label rpl16-rps3

Feature Metadata

label_name
rpl16-rps3
feature_type
igs
region
LSC
length_bp
1252
variable_sites
11
pi
0.00317
unique_haplotype_count
9
primer_available
yes
alignment_reliability
1.00
markerseek_score
58.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl16-rps3_p1 ACGGAATCTGGCTCTTTTGG TCCTCTACAAACCATTCGAGC 50.0 47.6 58.2 57.8 1387-1398 bp 1.0 70.5
rpl16-rps3_p2 ACGGAATCTGGCTCTTTTGG CCTCTACAAACCATTCGAGCT 50.0 47.6 58.2 57.7 1386-1397 bp 1.0 70.4
rpl16-rps3_p3 GAATCTGGCTCTTTTGGGGT TCCTCTACAAACCATTCGAGC 50.0 47.6 57.8 57.8 1384-1395 bp 1.0 70.1
rpl16-rps3_p4 GAATCTGGCTCTTTTGGGGT CCTCTACAAACCATTCGAGCT 50.0 47.6 57.8 57.7 1383-1394 bp 1.0 70.0
rpl16-rps3_p5 GTTCAAGTGCCTGAAGAGCA TCCTCTACAAACCATTCGAGC 50.0 47.6 58.4 57.8 1472-1483 bp 1.0 68.6