Hotspot

psaJ-rpl33

LSC · 72789-73251 · 463 bp

Back to Castanopsis

Pi 0.00513

MarkerSeek score 56.70

Primer available yes

Label psaJ-rpl33

Feature Metadata

label_name: psaJ-rpl33
feature_type: igs
region: LSC
length_bp: 463
variable_sites: 10
pi: 0.00513
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaJ-rpl33_p1	TTTATTTCCGGATGCGCTGA	GCCATGAACCCCCTTTGTAT	45.0	50.0	58.0	57.8	531-542 bp	1.0	81.4
psaJ-rpl33_p2	GTTTATTTCCGGATGCGCTG	GCCATGAACCCCCTTTGTAT	50.0	50.0	57.9	57.8	532-543 bp	1.0	80.8
psaJ-rpl33_p3	GATGCGCTGATATTCCCCTT	GCCATGAACCCCCTTTGTAT	50.0	50.0	57.8	57.8	521-532 bp	1.0	80.2
psaJ-rpl33_p4	TTCCGGATGCGCTGATATTC	GCCATGAACCCCCTTTGTAT	50.0	50.0	58.2	57.8	526-537 bp	1.0	80.1
psaJ-rpl33_p5	TTTCCGGATGCGCTGATATT	GCCATGAACCCCCTTTGTAT	45.0	50.0	57.7	57.8	527-538 bp	1.0	79.7