Hotspot

rbcL-accD

LSC · 61757-62331 · 575 bp

Back to Castanopsis

Pi 0.021

MarkerSeek score 49.00

Primer available yes

Label rbcL-accD

Feature Metadata

label_name: rbcL-accD
feature_type: igs
region: LSC
length_bp: 575
variable_sites: 25
pi: 0.021
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 49.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-accD_p1	ACAATTTGGCGGAGGAACTT	GTTAACTCACTACCAGCCGG	45.0	55.0	58.0	58.0	1508-1514 bp	0.909091	78.1
rbcL-accD_p2	CCGTACTACAATTTGGCGGA	GTTAACTCACTACCAGCCGG	50.0	55.0	58.0	58.0	1515-1521 bp	0.909091	78.1
rbcL-accD_p3	TCCGTACTACAATTTGGCGG	GTTAACTCACTACCAGCCGG	50.0	55.0	58.0	58.0	1516-1522 bp	0.909091	78.1
rbcL-accD_p4	AATGAGGGACGTGATCTTGC	GTTAACTCACTACCAGCCGG	50.0	55.0	58.0	58.0	1414-1420 bp	0.909091	78.0
rbcL-accD_p5	GCCGTAGCTAATCGAGTAGC	GTTAACTCACTACCAGCCGG	55.0	55.0	58.0	58.0	1459-1465 bp	0.909091	78.0