Hotspot

psbM-trnD-GUC

LSC · 32343-33589 · 1247 bp

Back to Castanopsis

Pi 0.00402

MarkerSeek score 60.20

Primer available yes

Label psbM-trnD-GUC

Feature Metadata

label_name: psbM-trnD-GUC
feature_type: igs
region: LSC
length_bp: 1247
variable_sites: 16
pi: 0.00402
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-trnD-GUC_p1	CGGAACTAGAATGAACAGTGC	TTCAATTGGTCAGAGCACCG	47.6	50.0	57.0	58.5	1355-1370 bp	1.0	62.0
psbM-trnD-GUC_p2	AGCAGTCGGAACTAGAATGA	TTCAATTGGTCAGAGCACCG	45.0	50.0	56.0	58.5	1361-1376 bp	1.0	61.9
psbM-trnD-GUC_p3	AGCAGTCGGAACTAGAATGAAC	TTCAATTGGTCAGAGCACCG	45.5	50.0	58.1	58.5	1361-1376 bp	1.0	60.8
psbM-trnD-GUC_p4	AGAATGAACAGTGCAGTAGC	TTCAATTGGTCAGAGCACCG	45.0	50.0	55.8	58.5	1348-1363 bp	1.0	60.2
psbM-trnD-GUC_p5	AGCAGTCGGAACTAGAATGAA	TTCAATTGGTCAGAGCACCG	42.9	50.0	56.8	58.5	1361-1376 bp	1.0	60.1