Hotspot

ndhF-rpl32

SSC · 118368-119313 · 946 bp

Back to Castanea

Pi 0.00606

MarkerSeek score 59.40

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 946
variable_sites: 11
pi: 0.00606
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 59.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	GCCGAAGATTTCTTGTTGCC	CTACCCAATACCCCTCCCTT	50.0	55.0	58.0	57.8	1133-1155 bp	1.0	80.2
ndhF-rpl32_p2	GCCGAAGATTTCTTGTTGCC	ACCCAATACCCCTCCCTTTT	50.0	50.0	58.0	58.2	1131-1153 bp	1.0	80.1
ndhF-rpl32_p3	CCGAAGATTTCTTGTTGCCG	CTACCCAATACCCCTCCCTT	50.0	55.0	57.8	57.8	1132-1154 bp	1.0	78.4
ndhF-rpl32_p4	CCGAAGATTTCTTGTTGCCG	ACCCAATACCCCTCCCTTTT	50.0	50.0	57.8	58.2	1130-1152 bp	1.0	78.3
ndhF-rpl32_p5	GCCGAAGATTTCTTGTTGCC	CCAATACCCCTCCCTTTTCC	50.0	55.0	58.0	57.5	1129-1151 bp	1.0	78.3