Hotspot

rps4-trnT-UGU

LSC · 51336-51674 · 339 bp

Back to Castanea

Pi 0.01

MarkerSeek score 57.20

Primer available yes

Label rps4-trnT-UGU

Feature Metadata

label_name: rps4-trnT-UGU
feature_type: igs
region: LSC
length_bp: 339
variable_sites: 7
pi: 0.01
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps4-trnT-UGU_p1	AAGATCGCTTCCGGCTTTAG	CTCAGAGGTTAGAGCATCGC	50.0	55.0	58.1	57.9	496-498 bp	1.0	79.9
rps4-trnT-UGU_p2	CTAAGATCGCTTCCGGCTTT	CTCAGAGGTTAGAGCATCGC	50.0	55.0	58.1	57.9	498-500 bp	1.0	79.9
rps4-trnT-UGU_p3	TTTCTAAGATCGCTTCCGGC	CTCAGAGGTTAGAGCATCGC	50.0	55.0	58.1	57.9	501-503 bp	1.0	79.9
rps4-trnT-UGU_p4	CCCCCAGACGTCGTATTTTT	CTCAGAGGTTAGAGCATCGC	50.0	55.0	57.9	57.9	449-451 bp	1.0	79.7
rps4-trnT-UGU_p5	AAGATCGCTTCCGGCTTTAG	GGTTCGATTCCGATAGCAGG	50.0	55.0	58.1	58.2	455-457 bp	1.0	79.4