Hotspot

ycf3-trnS-GGA

LSC · 49407-50335 · 929 bp

Back to Castanea

Pi 0.00194
MarkerSeek score 63.30
Primer available yes
Label ycf3-trnS-GGA

Feature Metadata

label_name
ycf3-trnS-GGA
feature_type
igs
region
LSC
length_bp
929
variable_sites
4
pi
0.00194
unique_haplotype_count
5
primer_available
yes
alignment_reliability
1.00
markerseek_score
63.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf3-trnS-GGA_p1 TTCTCCTGAAGTTGTCGGAA CCCTCGGTAAACAAAAGCCT 45.0 50.0 56.4 58.1 1074-1092 bp 1.0 68.7
ycf3-trnS-GGA_p2 TTTCTCCTGAAGTTGTCGGA CCCTCGGTAAACAAAAGCCT 45.0 50.0 56.4 58.1 1075-1093 bp 1.0 68.7
ycf3-trnS-GGA_p3 TTCTCCTGAAGTTGTCGGAA ACGGAAAGAGAGGGATTCGA 45.0 50.0 56.4 58.2 1095-1113 bp 1.0 68.2
ycf3-trnS-GGA_p4 TTTCTCCTGAAGTTGTCGGA ACGGAAAGAGAGGGATTCGA 45.0 50.0 56.4 58.2 1096-1114 bp 1.0 68.2
ycf3-trnS-GGA_p5 TTCTCCTGAAGTTGTCGGAA TTCCAATGCTACGCCTTCAA 45.0 45.0 56.4 57.8 1045-1063 bp 1.0 68.0