Hotspot

psbZ-trnG-UCC

LSC · 40643-41249 · 607 bp

Back to Castanea

Pi 0.00988
MarkerSeek score 53.30
Primer available yes
Label psbZ-trnG-UCC

Feature Metadata

label_name
psbZ-trnG-UCC
feature_type
igs
region
LSC
length_bp
607
variable_sites
15
pi
0.00988
unique_haplotype_count
5
primer_available
yes
alignment_reliability
1.00
markerseek_score
53.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbZ-trnG-UCC_p1 TTGCTTCTCCTGATGGTTGG TACCACTCAACTATATCCGC 50.0 45.0 57.8 54.1 737-751 bp 0.8 40.1
psbZ-trnG-UCC_p2 GCTTCTCCTGATGGTTGGTC TACCACTCAACTATATCCGC 55.0 45.0 58.3 54.1 735-749 bp 0.8 39.7
psbZ-trnG-UCC_p3 TTGCTTCTCCTGATGGTTGG TTACCACTCAACTATATCCGC 50.0 42.9 57.8 54.9 738-752 bp 0.8 39.0
psbZ-trnG-UCC_p4 GCTTCTCCTGATGGTTGGTC TTACCACTCAACTATATCCGC 55.0 42.9 58.3 54.9 736-750 bp 0.8 38.5
psbZ-trnG-UCC_p5 TTGCTTCTCCTGATGGTTGG TTTACCACTCAACTATATCCGC 50.0 40.9 57.8 55.7 739-753 bp 0.8 37.2