Hotspot

trnS-GCU-trnG-GCC

LSC · 9544-10567 · 1024 bp

Back to Castanea

Pi 0.0045
MarkerSeek score 65.00
Primer available yes
Label trnS-GCU-trnG-GCC

Feature Metadata

label_name
trnS-GCU-trnG-GCC
feature_type
igs
region
LSC
length_bp
1024
variable_sites
10
pi
0.0045
unique_haplotype_count
5
primer_available
yes
alignment_reliability
0.96
markerseek_score
65.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-GCU-trnG-GCC_p1 CAATCCGACGCTTTAGTCCA CGAAGGACCCCTTAACCATT 50.0 50.0 58.0 57.2 1084-1131 bp 1.0 77.7
trnS-GCU-trnG-GCC_p2 AATCCGACGCTTTAGTCCAC CGAAGGACCCCTTAACCATT 50.0 50.0 58.0 57.2 1083-1130 bp 1.0 77.7
trnS-GCU-trnG-GCC_p3 TAGTCCACTCAGCCATCTCC CGAAGGACCCCTTAACCATT 55.0 50.0 58.2 57.2 1071-1118 bp 1.0 76.0
trnS-GCU-trnG-GCC_p4 CAATCCGACGCTTTAGTCCA CGAAGGACCCCTTAACCATTT 50.0 47.6 58.0 57.9 1084-1131 bp 1.0 75.4
trnS-GCU-trnG-GCC_p5 AATCCGACGCTTTAGTCCAC CGAAGGACCCCTTAACCATTT 50.0 47.6 58.0 57.9 1083-1130 bp 1.0 75.4