Hotspot

trnN-GUU-trnR-ACG

Genome · 102148-102666 · 519 bp

Back to Cassytha

Pi 0.0253

MarkerSeek score 65.90

Primer available yes

Label trnN-GUU-trnR-ACG

Feature Metadata

label_name: trnN-GUU-trnR-ACG
feature_type: igs
region: Genome
length_bp: 519
variable_sites: 12
pi: 0.0253
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.91
markerseek_score: 65.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnN-GUU-trnR-ACG_p1	GCTGTTAACTGACTGGTCGT	CAGAGGATTAGAGCACGTGG	50.0	55.0	57.8	57.8	624-628 bp	1.0	82.6
trnN-GUU-trnR-ACG_p2	TGGTCGTAGGTTCGAATCCT	CAGAGGATTAGAGCACGTGG	50.0	55.0	58.2	57.8	611-615 bp	1.0	82.6
trnN-GUU-trnR-ACG_p3	CTGGTCGTAGGTTCGAATCC	CAGAGGATTAGAGCACGTGG	55.0	55.0	57.8	57.8	612-616 bp	1.0	82.2
trnN-GUU-trnR-ACG_p4	TTCCTCAGTAGCTCAGTGGT	CAGAGGATTAGAGCACGTGG	50.0	55.0	57.7	57.8	654-658 bp	1.0	81.2
trnN-GUU-trnR-ACG_p5	GGTTCGAATCCTACTTGGGG	CAGAGGATTAGAGCACGTGG	55.0	55.0	57.7	57.8	603-607 bp	1.0	81.2