Hotspot

psaC-rps15

Genome · 94445-95708 · 1264 bp

Back to Cassytha

Pi 0.0183

MarkerSeek score 57.50

Primer available yes

Label psaC-rps15

Feature Metadata

label_name: psaC-rps15
feature_type: igs
region: Genome
length_bp: 1264
variable_sites: 23
pi: 0.0183
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 57.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaC-rps15_p1	ATACATCTGTGGGACAGGCT	TGAGTTAGGTATTCGAGAACCA	50.0	40.9	58.1	56.3	1369-1374 bp	1.0	51.4
psaC-rps15_p2	ACATCCGTCCCAAGGTATCA	TGAGTTAGGTATTCGAGAACCA	50.0	40.9	58.1	56.3	1395-1400 bp	1.0	51.3
psaC-rps15_p3	GACAGGCTCGTACACATTGA	TGAGTTAGGTATTCGAGAACCA	50.0	40.9	57.6	56.3	1357-1362 bp	1.0	49.4
psaC-rps15_p4	ACAGGCTCGTACACATTGAG	TGAGTTAGGTATTCGAGAACCA	50.0	40.9	57.6	56.3	1356-1361 bp	1.0	49.4
psaC-rps15_p5	CAGGCTCGTACACATTGAGT	TGAGTTAGGTATTCGAGAACCA	50.0	40.9	57.6	56.3	1355-1360 bp	1.0	49.4