Hotspot

ccsA-psaC

Genome · 92480-94198 · 1719 bp

Back to Cassytha

Pi 0.00819

MarkerSeek score 63.60

Primer available yes

Label ccsA-psaC

Feature Metadata

label_name: ccsA-psaC
feature_type: igs
region: Genome
length_bp: 1719
variable_sites: 14
pi: 0.00819
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 63.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA-psaC_p1	GGGTGTGGATTCGGCAATTA	GAATCTGCTTGTCCAACCGA	50.0	50.0	58.2	58.2	1913-1915 bp	1.0	77.5
ccsA-psaC_p2	GGGTGTGGATTCGGCAATTA	CCAAGAACCGAAGACTGTGT	50.0	50.0	58.2	57.8	1949-1951 bp	1.0	77.1
ccsA-psaC_p3	GGGTGTGGATTCGGCAATTA	TCTGCTTGTCCAACCGATTT	50.0	45.0	58.2	57.7	1910-1912 bp	1.0	76.9
ccsA-psaC_p4	GGGTGTGGATTCGGCAATTA	TGAATCTGCTTGTCCAACCG	50.0	50.0	58.2	58.5	1914-1916 bp	1.0	75.2
ccsA-psaC_p5	GGGTGTGGATTCGGCAATTA	ATCTGCTTGTCCAACCGATT	50.0	45.0	58.2	57.5	1911-1913 bp	1.0	75.1