Hotspot

trnI-CAU-ycf2

Genome · 83121-83200 · 80 bp

Back to Cassytha

Pi 0.0625

MarkerSeek score 82.80

Primer available yes

Label trnI-CAU-ycf2

Feature Metadata

label_name: trnI-CAU-ycf2
feature_type: igs
region: Genome
length_bp: 80
variable_sites: 5
pi: 0.0625
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 82.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnI-CAU-ycf2_p1	GTTGGGCGCTTTAACCATTC	CACCCAATCAAATTTGGCCC	50.0	50.0	58.0	57.9	208 bp	1.0	81.0
trnI-CAU-ycf2_p2	GTTGGGCGCTTTAACCATTC	CCACCCAATCAAATTTGGCC	50.0	50.0	58.0	57.9	209 bp	1.0	80.9
trnI-CAU-ycf2_p3	TCATTGGTGTGCATCCAACA	CACCCAATCAAATTTGGCCC	45.0	50.0	58.0	57.9	259 bp	1.0	80.6
trnI-CAU-ycf2_p4	TCATTGGTGTGCATCCAACA	CCACCCAATCAAATTTGGCC	45.0	50.0	58.0	57.9	260 bp	1.0	80.6
trnI-CAU-ycf2_p5	TCATTGGTGTGCATCCAACA	CCCACCCAATCAAATTTGGC	45.0	50.0	58.0	57.9	261 bp	1.0	80.6