Hotspot

rbcL-accD

Genome · 53289-53969 · 681 bp

Back to Cassytha

Pi 0.0269

MarkerSeek score 76.40

Primer available yes

Label rbcL-accD

Feature Metadata

label_name: rbcL-accD
feature_type: igs
region: Genome
length_bp: 681
variable_sites: 17
pi: 0.0269
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 76.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-accD_p1	ATCAAATTCGAATTCGCCGC	ATTGAGCCAACGTTTTCCCA	45.0	45.0	57.9	58.3	688-738 bp	1.0	82.7
rbcL-accD_p2	ATCAAATTCGAATTCGCCGC	TTGAGCCAACGTTTTCCCAT	45.0	45.0	57.9	58.3	687-737 bp	1.0	82.7
rbcL-accD_p3	GCTGCTTGTGAGGTATGGAA	ATTGAGCCAACGTTTTCCCA	50.0	45.0	57.9	58.3	712-762 bp	1.0	82.5
rbcL-accD_p4	GCTGCTTGTGAGGTATGGAA	TTGAGCCAACGTTTTCCCAT	50.0	45.0	57.9	58.3	711-761 bp	1.0	82.5
rbcL-accD_p5	ATCAAATTCGAATTCGCCGC	ATTTACTTAGCCCACACCCG	45.0	50.0	57.9	57.6	737-787 bp	1.0	81.6