Hotspot

atpF-atpH

Genome · 13021-13451 · 431 bp

Back to Cassytha

Pi 0.0139

MarkerSeek score 62.90

Primer available yes

Label atpF-atpH

Feature Metadata

label_name: atpF-atpH
feature_type: igs
region: Genome
length_bp: 431
variable_sites: 6
pi: 0.0139
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF-atpH_p1	TTAAACCCGAAACTCCCAGC	CCCGAAGCAGAGGGTAAAAT	50.0	50.0	58.1	57.6	619-622 bp	1.0	76.6
atpF-atpH_p2	TTAAACCCGAAACTCCCAGC	ATTTATGGGTTGGTCGTGGC	50.0	50.0	58.1	58.5	553-556 bp	1.0	75.8
atpF-atpH_p3	GGCCAGTGACCAAAGGAAAT	CCCGAAGCAGAGGGTAAAAT	50.0	50.0	58.4	57.6	595-598 bp	1.0	74.5
atpF-atpH_p4	TTAAACCCGAAACTCCCAGC	ACGATTTATGGGTTGGTCGT	50.0	45.0	58.1	57.2	556-559 bp	1.0	73.9
atpF-atpH_p5	GGCCAGTGACCAAAGGAAAT	ATTTATGGGTTGGTCGTGGC	50.0	50.0	58.4	58.5	529-532 bp	1.0	73.7