Hotspot

ycf1

IRb · 115006-115807 · 802 bp

Pi 0.0312

MarkerSeek score 76.70

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: IRb
length_bp: 802
variable_sites: 25
pi: 0.0312
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 76.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	AGAAAAGAAAAAGGGAGGTTTGTG	GTAGGGGGTGGTCGAATTTC	37.5	55.0	58.0	58.0	902-1152 bp	1.0	49.5
ycf1_p2	AGAAAAGAAAAAGGGAGGTTTGTG	TAGGGGGTGGTCGAATTTCT	37.5	50.0	58.0	58.0	901-1151 bp	1.0	49.5
ycf1_p3	AAGAAAAAGGGAGGTTTGTG	GTAGGGGGTGGTCGAATTTC	40.0	55.0	53.9	58.0	898-1148 bp	1.0	48.7
ycf1_p4	AAGAAAAAGGGAGGTTTGTG	TAGGGGGTGGTCGAATTTCT	40.0	50.0	53.9	58.0	897-1147 bp	1.0	48.7
ycf1_p5	AAAGAAAAAGGGAGGTTTGTG	GTAGGGGGTGGTCGAATTTC	38.1	55.0	54.8	58.0	899-1149 bp	1.0	47.7