Hotspot

ndhG-ndhI

SSC · 124061-124449 · 389 bp

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Pi 0.0315
MarkerSeek score 74.40
Primer available yes
Label ndhG-ndhI

Feature Metadata

label_name
ndhG-ndhI
feature_type
igs
region
SSC
length_bp
389
variable_sites
12
pi
0.0315
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.98
markerseek_score
74.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhG-ndhI_p1 TCCCAACGAAAAGGCAGAAA TCAAATTGCTTTGGGTCGTT 45.0 40.0 57.9 56.4 582-611 bp 1.0 69.7
ndhG-ndhI_p2 TCCCAACGAAAAGGCAGAAA TCGACTTATGATCGTCACGA 45.0 45.0 57.9 56.2 614-643 bp 1.0 67.9
ndhG-ndhI_p3 TCCCAACGAAAAGGCAGAAA ATCAAATTGCTTTGGGTCGT 45.0 40.0 57.9 56.2 583-612 bp 1.0 67.9
ndhG-ndhI_p4 TCCCAACGAAAAGGCAGAAA TCAAATTGCTTTGGGTCGTTT 45.0 38.1 57.9 57.2 582-611 bp 1.0 67.7
ndhG-ndhI_p5 TCCCAACGAAAAGGCAGAAA TCGACTTATGATCGTCACGAA 45.0 42.9 57.9 57.0 614-643 bp 1.0 65.9