Hotspot

ndhE-ndhG

SSC · 123294-123529 · 236 bp

Pi 0.0297

MarkerSeek score 69.60

Primer available yes

Label ndhE-ndhG

Feature Metadata

label_name: ndhE-ndhG
feature_type: igs
region: SSC
length_bp: 236
variable_sites: 7
pi: 0.0297
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 69.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhE-ndhG_p1	TCGAACCATATTTCGGCTCG	AGCTGCTTTGATAGGTGCAA	50.0	45.0	58.2	57.8	363 bp	1.0	78.7
ndhE-ndhG_p2	CACATAAGGGCTCGAACCAT	AGCTGCTTTGATAGGTGCAA	50.0	45.0	57.7	57.8	374 bp	1.0	77.2
ndhE-ndhG_p3	TTTCGGCTCGTGATCAATCC	AGCTGCTTTGATAGGTGCAA	50.0	45.0	58.3	57.8	353 bp	1.0	77.2
ndhE-ndhG_p4	GACACATAAGGGCTCGAACC	AGCTGCTTTGATAGGTGCAA	55.0	45.0	58.3	57.8	376 bp	1.0	77.1
ndhE-ndhG_p5	GCTCGAACCATATTTCGGCT	AGCTGCTTTGATAGGTGCAA	50.0	45.0	58.4	57.8	365 bp	1.0	76.6