Hotspot

rps3-rps19

LSC · 88917-89540 · 624 bp

Back to Cassia

Pi 0.0263
MarkerSeek score 69.40
Primer available yes
Label rps3-rps19

Feature Metadata

label_name
rps3-rps19
feature_type
igs
region
LSC
length_bp
624
variable_sites
16
pi
0.0263
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.98
markerseek_score
69.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps3-rps19_p1 TACCAAGTCTGAAACCGAGC ACTCTAAACTTCCGGGGACA 50.0 50.0 57.5 58.0 709-715 bp 1.0 79.7
rps3-rps19_p2 GTCTGAAACCGAGCGGATTT ACTCTAAACTTCCGGGGACA 50.0 50.0 58.6 58.0 703-709 bp 1.0 78.9
rps3-rps19_p3 TGGTTGTGCAAACCAAAGAG ACTCTAAACTTCCGGGGACA 45.0 50.0 57.0 58.0 747-753 bp 1.0 75.5
rps3-rps19_p4 TCTGAAACCGAGCGGATTTA ACTCTAAACTTCCGGGGACA 45.0 50.0 57.0 58.0 702-708 bp 1.0 75.0
rps3-rps19_p5 TACCAAGTCTGAAACCGAGC CTAAACTTCCGGGGACATGC 50.0 55.0 57.5 58.6 706-712 bp 1.0 74.9