Hotspot

rpl16-rps3

LSC · 88105-88259 · 155 bp

Back to Cassia

Pi 0.0387
MarkerSeek score 74.80
Primer available yes
Label rpl16-rps3

Feature Metadata

label_name
rpl16-rps3
feature_type
igs
region
LSC
length_bp
155
variable_sites
6
pi
0.0387
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
74.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl16-rps3_p1 ACAACGAGTCGCACACTAAG CCTCTACAAACCATTCGAGCA 50.0 47.6 58.2 58.0 283 bp 1.0 71.6
rpl16-rps3_p2 ACAACGAGTCGCACACTAAG TCCTCTACAAACCATTCGAGC 50.0 47.6 58.2 57.8 284 bp 1.0 69.9
rpl16-rps3_p3 CACAACGAGTCGCACACTAA CCTCTACAAACCATTCGAGCA 50.0 47.6 58.5 58.0 284 bp 1.0 69.3
rpl16-rps3_p4 CACAACGAGTCGCACACTAA TCCTCTACAAACCATTCGAGC 50.0 47.6 58.5 57.8 285 bp 1.0 67.6
rpl16-rps3_p5 AAAACCAACACAACGAGTCG CCTCTACAAACCATTCGAGCA 45.0 47.6 57.2 58.0 292 bp 1.0 66.9